17 cetoesteroides urinarios

total urinary neutral 17-steroids were determined in normal and in castrated horses. one liter of a 15-26 hours urine collection was hydrolysed by refluxing with 10% hc1 (v/v) for ten minutes and extracted with peroxyde-free ethyl ether. the extract was purified by washing with saturated nahco3 and koh solutions. one half of the crude neutral fraction was fractionated with girard's "t" reagent . the zimmermann reaction was performed both in the ketonic and in the crude neutral extracts, using alcoholic koh and a 60 minutes period for the colour development in the dark. optical density measuments were made in a grating coleman universal spectrophotometer at 420 mμ and 520mμ; for the crude neutral fraction a colour correction equation was applied. the aliquot fraction used for colorimety was adjusted for keeping optical density measurements within the range to . androsterone (mp. 184-°c) with an absorption maximum at mμ (beckman model du spectrophotometer) was used as a reference standard. table i, ilustrates the results obtained. at the probability level there is a significant difference among castrated and normal group means (fischer's "t" test.) when were used the data obtained from the ketonic fractions; in spite of the use of a colour correction applied for inespecific chromogens, the same results could not be obtained with the crude neutral fractions, since girard's reagent fractionation is generaly accepted as the best method for correcting the inespecific chromogen interference in the determination of the 17-ketosteroids by the zimmermann reaction, we emphasize the value of the results obtained with the ketonic fractions. from these results it appears, as occurs in others mammals, that castrated horses show a lower level of urinary 17-ketosteroids excretion than the normal horses. the significance of the horse testis contribution for the neutral urinary steroid metabolites is discussed. since horse urine has a low androgenic activit

17 cetoesteroides urinarios

17 cetoesteroides urinarios

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